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        Fully Human Therapeutic MAb Discovery
        • ChemPartner’s proprietary fully human na?ve phage library with diversity ~10^11. Efficient?library?selection and screening?with high-throughput robotics.
        • All V-genes were amplified with high fidelity from diverse healthy donors. Stringent cloning procedures ensure a high percentage of functional clones in the library.
        • Independent VH, VL libraries maximize V-gene recovery and facilitate subsequent affinity maturation with chain shuffling
        • All V-gene families are individually cloned and assembled, maximizing library diversity and reducing gene bias due to PCR cloning.
        • First major na?ve?antibody?library built with predefined V-gene composition to match the human immune repertoire

        CP na?ve library V-gene composition matches the natural repertoire

        Affinity Maturation
        • Kd improvement to sub-nM to pM;? Binding kinetics (off-rate) improvement
        • Both scFv and Fab formats may be used to generate CDR mutagenesis libraries
        • Libraries are panned against biotinylated Ag at decreasing concentrations in solution
        • To select for pM binders, off-rate selection method is employed. Unlabeled antigen is used for competition
        • Enriched clones are produced in 96-well format, and final ranking with high throughput screening robotics
        • Best clones identified for full IgG conversion and extensive characterization by Biacore and bioassays
        • Standard timeline with full IgG delivery:? 3 months for final KD~nM;? 4-5 months for final KD~pM

         

        Comprehensive Drug Property Optimization of MAb Candidates
        • Specificity, cross-reactivity
        • Germlining, immunogenicity reduction
        • Drug stability (thermal, chemical & proteolytic)
        • Expressional level, solubility, manufacturability
        • IgG reformatting, Fc engineering, antibody fragments, fusion proteins, scaffolds

         

        CP HTP Screening Platform: semi-rational mutagenesis library, automatic screen with two Tecan Freedom EVO Workstations, up to ~10^4 variants per day ;

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